反应详情

(2E)-3-(6-{[(3R)-1-Benzyl-3-pyrrolidinyl]amino}-3-pyridyl)-N-hydroxyacrylamide dihydrochloride (860 mg) was dissolved into water (40 ml), and the solution was adjusted to ca. pH4.0 by addition of a few drops of aq NaHCO3 solution. The aqueous solution was chromatographed with HP-20(100 ml), washing with water and eluting with 75% aqueous MeOH. The elute was concentrated to ca. 10 ml in vacuo, and the resulting precipitate was collected by filtration. (2E)-3-(6-{[(3R)-1-benzyl-3-pyrrolidinyl]amino}-3-pyridyl)-N-hydroxyacrylamide (240 mg) was obtained as colorless powder.

1. customThe aqueous solution was chromatographed with HP-20(100 ml)
2. washwashing with water
3. washeluting with 75% aqueous MeOH
4. concentrationThe elute was concentrated to ca. 10 ml in vacuo
5. filtrationthe resulting precipitate was collected by filtration