Linoleic Acid → Stearidonic Acid

HRID 2265162Notes

Reaction equation

HRID 2265162 reaction equation
[C:1]([OH:20])(=[O:19])[CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8]/[CH:9]=[CH:10]\[CH2:11]/[CH:12]=[CH:13]\[CH2:14][CH2:15][CH2:16][CH2:17][CH3:18].C(O)(=O)CCCC/C=C\C/C=C\C/C=C\CCCCC.CC/C=C\C/C=C\C/C=C\CCCCCCCC(O)=O>>[C:1]([OH:20])(=[O:19])[CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH2:11]/[CH:12]=[CH:13]\[CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH3:18]

Participants

4

Reactant 3

HCID 5280450Linoleic AcidHCID 5280933Gamma-Linolenic acidHCID 5280934Linolenic Acid

Product 1

HCID 5312508Stearidonic Acid

Procedure

FIG. 23 shows the results of the feeding experiments with the yeast cells which comprise plasmid pYES52Dest-MaLPAAT (MaB4_AT). In FIG. 23, A/B, the yeast cultures were fed linoleic acid (18:2 Δ9,12). In comparison with the control culture (FIG. 23, A), the yeast cells with the MaLPAAT showed a markedly higher conversion (increased 4-fold) of 18:2 into γ-linolenic acid (18:3 Δ6,9,12), and a 3.5-fold increase of the fatty acid 20:2 Δ11,14 elongated from 18:2. Analogously, when feeding linolenic acid (18:3 Δ9, 12, 15), a markedly higher conversion rate to give stearidonic acid (18:4 Δ6, 9, 12, 15) and isoarachidonic acid (20:4 Δ8, 11, 14, 17) was observed in comparison with the controls (FIG. 24).

Workup

  1. customresults of the feeding experiments with the yeast cells which

Sources & evidence

ORD record
ord-030b86e0259f4917aacfdf768cc62fe3
Source dataset
uspto-grants-2010_12
Patent number
US07855321B2

Reaction notes

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