Linoleic Acid → Stearidonic Acid

Participants

Reaction Steps

FIG. 23 shows the results of the feeding experiments with the yeast cells which comprise plasmid pYES52Dest-MaLPAAT (MaB4_AT). In FIG. 23, A/B, the yeast cultures were fed linoleic acid (18:2 Δ9,12). In comparison with the control culture (FIG. 23, A), the yeast cells with the MaLPAAT showed a markedly higher conversion (increased 4-fold) of 18:2 into γ-linolenic acid (18:3 Δ6,9,12), and a 3.5-fold increase of the fatty acid 20:2 Δ11,14 elongated from 18:2. Analogously, when feeding linolenic acid (18:3 Δ9, 12, 15), a markedly higher conversion rate to give stearidonic acid (18:4 Δ6, 9, 12, 15) and isoarachidonic acid (20:4 Δ8, 11, 14, 17) was observed in comparison with the controls (FIG. 24).

Provenance & Evidence