反应详情

6-Amino-9-benzyl-2-chloropurine (10 mg, 0.385 mmol) and propylamine (228 mg, 3.85 mmol) in methanol (50 ml) were heated at 120° C. for 10 hours in autoclave. The reaction mixture was condensed in vacuo and to the residue was added 5N aqueous sodium hydroxide, followed by extraction with chloroform. The organic layer was dried on sodium sulfate, filtered and the solvent in the filtrate was evaporated in vacuo. The residue was purified with silica gel chromatography (2% methanol/chloroform) to give the subject compound (99 mg, yield 91%).

1. customcondensed in vacuo
2. extractionfollowed by extraction with chloroform
3. customThe organic layer was dried on sodium sulfate
4. filtrationfiltered
5. customthe solvent in the filtrate was evaporated in vacuo
6. customThe residue was purified with silica gel chromatography (2% methanol/chloroform)