Water → HCID 178437364, compound

HRID 2271260Notes

Reaction equation

HRID 2271260 reaction equation
[CH2:1](O)[C:2](N)(CO)CO.Cl.[C@@H:10]1([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23](N)[C:22]=3[N:21]=[CH:20]2)[O:18][C@H:15]([CH2:16][OH:17])[C@@H:13]([OH:14])[C@H:11]1O.[OH2:29]>>[C:1]([C@:15]1([CH2:16][OH:17])[O:18][C@@H:10]([N:19]2[C:28]3[N:27]=[CH:26][N:25]=[C:23]([OH:29])[C:22]=3[N:21]=[CH:20]2)[CH2:11][C@@H:13]1[OH:14])#[CH:2]

Participants

4

Reactant 3

HCID 962WaterHCID 60961AdenosineHCID 93573Tromethamine hydrochloride

Product 1

HCID 178437364HCID 178437364, compound

Conditions

Temperature
40 °C

Procedure

To a Tris-HCl buffer solution (6 ml, pH 7.5) of Compound 23 (0.022 g, 0.08 mmol), adenosine deaminase (0.044 ml, 20 unit) was added, and the mixture was stirred for 2.5 hours at 40° C., followed by cooling to room temperature. The reaction mixture was applied to a reverse-phase ODS silica gel column (50 g), desalted by water (500 ml) flow, and through use of a 2.5% aqueous ethanol, Compound 29 was eluted. Subsequently, the Compound 29 was pulverized with isopropanol, to thereby yield 0.016 g of Compound 29 (72%).

Workup

  1. temperatureby cooling to room temperature
  2. washthrough use of a 2.5% aqueous ethanol, Compound 29 was eluted

Sources & evidence

ORD record
ord-bcbb280f387a46459cdcad90bd5522ce
Source dataset
uspto-grants-2001_12
Patent number
US06333315B1

Reaction notes

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