Bicarbonate Ion → HCID 71237586, Verbindung

HRID 2280734Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 2280734
Cl[C:2]1[N:7]=[C:6]([NH:8][C@@H:9]2[CH2:14][CH2:13][CH2:12][C@H:11]([OH:15])[CH2:10]2)[C:5]([C:16]#[N:17])=[CH:4][N:3]=1.[OH-].[NH4+].C(O)(C(F)(F)F)=O.C(=O)(O)[O-]>C(O)C.[Ni]>[NH2:17][CH2:16][C:5]1[C:6]([NH:8][C@@H:9]2[CH2:14][CH2:13][CH2:12][C@H:11]([OH:15])[CH2:10]2)=[N:7][CH:2]=[N:3][CH:4]=1

Komponenten

7

Edukt 4

HCID 769Bicarbonate IonHCID 6422Trifluoroacetic acidHCID 14923Ammonium HydroxideHCID 86676341HCID 86676341, Verbindung

Produkt 1

HCID 71237586HCID 71237586, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 935NickelKatalysatorHCID 702EthanolLösungsmittel

Versuchsvorschrift

To a mixture of 2-chloro-4-((1R,3S)-3-hydroxycyclohexyl-amino)pyrimidine-5-carbonitrile (70 mg, 0.277 mmol) and ammonium hydroxide (500 μl, 12.84 mmol) in ethanol (4 mL) was added Raney nickel (16.26 mg, 0.277 mmol). The resulting mixture was evacuated and then stirred under a balloon of hydrogen at atmospheric pressure and ambient temperature overnight. Ethanol was removed in vacuo and the remaining residue was purified using reverse-phased preparative HPLC (5-80% acetonitrile+0.1% TFA in water+0.1% TFA, over 30 min). Fractions containing product were concentrated under reduced pressure. The resulting residue was redissolved in methanol (5 mL), passed over a Varian StratoSpheres HCO3 resin SPE tube for TFA removal (0.9 mmol bicarbonate equiv.), and then concentrated under reduced pressure to afford the title compound as an oil that was used for NMR purposes only. The regiochemistry was confirmed by the presence of two distinct aromatic peaks in the 1H NMR spectrum and the observation of an NOE signal between the C5 methylene hydrogens and only one of these aromatic peaks in the NOISY spectrum. 1H NMR (400 MHz, DMSO-d6) δ ppm 8.32 (s, 1H), 7.92 (s, 1H), 7.32 (d, J=7.48 Hz, 1H), 4.67 (d, J=3.87 Hz, 1H), 3.88-4.01 (m, 1H), 3.59 (s, 2H), 3.49 (d, J=3.61 Hz, 1H), 2.06-2.16 (m, 1H), 1.99 (br. s., 2H), 1.81 (t, J=13.28 Hz, 2H), 1.69 (dt, J=13.35, 3.51 Hz, 1H), 1.01-1.35 (m, 4H). MS (ESI) m/z 223.5 [M+1]+.

Aufarbeitung

  1. customThe resulting mixture was evacuated
  2. customEthanol was removed in vacuo
  3. customthe remaining residue was purified
  4. customreverse-phased preparative HPLC (5-80% acetonitrile+0.1% TFA in water+0.1% TFA, over 30 min)
  5. additionFractions containing product
  6. concentrationwere concentrated under reduced pressure
  7. dissolutionThe resulting residue was redissolved in methanol (5 mL)
  8. concentrationconcentrated under reduced pressure

Quellen und Nachweise

ORD-Eintrag
ord-485aa900ba60442c86af9531cda7a268
Quelldatensatz
uspto-grants-2015_09
Patentnummer
US09139534B2

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