HCID 131721445, compound → Hippuric Acid

HRID 2304369Notes

Reaction equation

HRID 2304369 reaction equation
COC1CC2[C:6]3([C:18]4[C:13](=CC(OC)=C(OC)C=4O)CN2CC3)CC1.N[C@H:25]([C:30]([OH:32])=O)[CH2:26][C:27](=O)O.COC1CC2C3(C4C(=CC(OC)=C(OC)C=4O)CN2CC3)CC1.[NH2:56][C@H:57]([C:63]([OH:65])=[O:64])CCC(=O)O>>[C:63]([OH:65])(=[O:64])[CH2:57][NH:56][C:30]([C:25]1[CH:26]=[CH:27][CH:6]=[CH:18][CH:13]=1)=[O:32]

Participants

3

Reactant 2

HCID 131721445HCID 131721445, compoundHCID 131721446HCID 131721446, compound4×

Product 1

HCID 464Hippuric Acid

Procedure

D253K HCPB (4 μg/ml for Hipp-Asp and 0.5 μg/ml for Hipp-Glu), [Q54R,D145A,D253K]HCPB (1.5 μg/ml for Hipp-Glu), [G251N,D253K]HCPB (0.14 μg/ml for Hipp-Glu) and [G251T,D253K]HCPB (0.02 μg/ml for Hipp-Glu) were added to these substrates (500 μl reaction volume) to start the reaction. Samples were incubated for 5 h at 37° C. Reactions were terminated by the addition of 500 μl methanol/distilled water (80/20) containing 0.2% TFA. The amount of hippuric acid produced was quantified by HPLC as described in Example 6.

Workup

  1. customthe reaction
  2. customReactions were terminated by the addition of 500 μl methanol/
  3. distillationdistilled water (80/20)
  4. additioncontaining 0.2% TFA

Sources & evidence

ORD record
ord-ef6d0f066a8d4c1c8a21434d4dd020f9
Source dataset
uspto-grants-2002_08
Patent number
US06436691B1

Reaction notes

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