HCID 131721445, compound → Hippuric Acid

Participants

Reaction Steps

D253K HCPB (4 μg/ml for Hipp-Asp and 0.5 μg/ml for Hipp-Glu), [Q54R,D145A,D253K]HCPB (1.5 μg/ml for Hipp-Glu), [G251N,D253K]HCPB (0.14 μg/ml for Hipp-Glu) and [G251T,D253K]HCPB (0.02 μg/ml for Hipp-Glu) were added to these substrates (500 μl reaction volume) to start the reaction. Samples were incubated for 5 h at 37° C. Reactions were terminated by the addition of 500 μl methanol/distilled water (80/20) containing 0.2% TFA. The amount of hippuric acid produced was quantified by HPLC as described in Example 6.

Provenance & Evidence