4-Methylmorpholine → HCID 12050384, compound

HRID 2305018Notes

Reaction equation

HRID 2305018 reaction equation
[OH:1][C:2]([CH2:18][S:19]([C:22]1[CH:27]=[CH:26][C:25]([O:28][CH3:29])=[CH:24][CH:23]=1)(=[O:21])=[O:20])([CH2:6][NH:7][C:8]([C:10]1[CH:15]=[CH:14][C:13]([O:16][CH3:17])=[CH:12][CH:11]=1)=[O:9])[C:3](O)=[O:4].Cl.C([O:35][NH2:36])(C)(C)C.CN1CCOCC1>C(Cl)Cl>[OH:35][NH:36][C:3](=[O:4])[C:2]([OH:1])([CH2:18][S:19]([C:22]1[CH:27]=[CH:26][C:25]([O:28][CH3:29])=[CH:24][CH:23]=1)(=[O:21])=[O:20])[CH2:6][NH:7][C:8]([C:10]1[CH:15]=[CH:14][C:13]([O:16][CH3:17])=[CH:12][CH:11]=1)=[O:9]

Participants

5

Reactant 3

HCID 79724-MethylmorpholineHCID 2777906O-tert-butylhydroxylamine hydrochlorideHCID 18459286HCID 18459286, compound

Product 1

HCID 12050384HCID 12050384, compoundYield 67.5%

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

2-Hydroxy-2-[(4-methoxybenzenesulfonyl)methyl]-3-(N-(4-methoxybenzenecarbonyl)amino)-propionic acid (100 mg, 0.24 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiilnide hydrochloride (68 mg, 0.35 mmol), O-tert-butylhydroxylamine hydrochloride (118 mg, 0.944 mmol), and 4-methylmorpholine (131 mg, 0.354 mmol) are dissolved in 20 mL of methylene chloride. The reaction mixture is stirred under nitrogen for 6 hours. The solvent is removed under reduced pressure, and the residue is dissolved in ethyl acetate. The organic layer is washed with 1N sodium hydrogen sulfate, 5% sodium bicarbonate, and saturated brine and dried over magnesium sulfate. The solvent is removed to yield 71 mg of white solid which is recrystallized from methanol. The tert-butyl protecting group is removed by treatment with 50% trifluoroacetic acid in methylene chloride for 24 hours. The solvents are removed, and the crude product is purified by reverse phase chromatography on a C18 Vydac column using a water-acetonitrile elution system to yield the title compound as a white solid.

Workup

  1. customThe solvent is removed under reduced pressure
  2. dissolutionthe residue is dissolved in ethyl acetate
  3. washThe organic layer is washed with 1N sodium hydrogen sulfate, 5% sodium bicarbonate, and saturated brine
  4. dry with materialdried over magnesium sulfate
  5. customThe solvent is removed

Sources & evidence

ORD record
ord-d0bffbe9ceb04f78b4b6ec6dd34b57d5
Source dataset
uspto-grants-2002_08
Patent number
US06437177B1

Reaction notes

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