Acetic Acid → Ninhydrin

HRID 2307525Notes

Reaction equation

HRID 2307525 reaction equation
[CH2:1]([OH:5])[CH2:2][CH2:3][CH3:4].[C:6]([OH:9])(=[O:8])[CH3:7].C(O)(=[O:12])C.[CH2:14](O)[CH2:15][CH2:16]C>O>[CH:15]1[CH:16]=[C:2]2[C:1]([C:6]([OH:9])([OH:8])[C:7](=[O:12])[C:3]2=[CH:4][CH:14]=1)=[O:5]

Participants

5

Reactant 3

HCID 176Acetic AcidHCID 2631-ButanolHCID 22035215Acetic acid--butan-1-ol (1/1)

Product 1

HCID 10236Ninhydrin

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

Thin glass plated (20×20 cm) coated (0.4 mm to 0.5 mm thick) with silica gel G (Kieselgel G nach Stahl; E. Merck) were activated at 100° C. for half an hour. The solution containing the isolated substance was applied 1 cm above the edge of the plates were run in an organic solvent mixture of n-butanol and acetic acid and water. It should be n-butanol acetic acid and water (12:5:2). The developed plates were dried at room temperature and sprayed with 0.25% nin-hydrin in acetone. The nin-hydrin positive spots (R=0.19) of the isolate nearly corresponding to insulin were collected from about 200 unsprayed plates along with the silica gel G and extracted with 50% ethanol buffered with ammonium hydroxide/10% formic acid. The extract was filtered and dried in vacuo. Pure colorless crystals thus obtained were weighed (3 g/100 gram dry weight of seeds).

Workup

  1. waitwere activated at 100° C. for half an hour
  2. additionThe solution containing the isolated substance
  3. customThe developed plates were dried at room temperature

Sources & evidence

ORD record
ord-f39d1a109c8b476d8e6ea573a9713008
Source dataset
uspto-grants-2004_12
Patent number
US06831162B2

Reaction notes

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