Trisodium Phosphate → Pseudoephedrine

Participants

Reaction Conditions

Reaction Steps

Microbacterium arborescens IFO 3750 was inoculated to a medium (5 ml) containing 1% glucose, 0.5% peptone, and 0.3% yeast extract, and shake-culturing was carried out at 30° C. for 48 hours. After the cultured solution was centrifuged to give bacterial cells, the cells were placed into a test tube. To this was added 0.1 M sodium phosphate buffer (pH 7.0, 1 ml) and suspended. To this was added dl-2-methylamino-1-phenyl-1-propanone hydrochloride (1 mg) and reaction was allowed to take place under shaking at 30° C. for 24 hours. After the reaction, the reaction solution was centrifuged to remove the bacterial cells and the supernatant was subjected to HPLC to give optically active pseudoephedrine: μ Bondapakphenyl manufactured by Waters Inc.; diameter of 4 mm; length of 300 mm; eluent-0.05 M sodium phosphate buffer (containing 7% acetonitrile); pH 5.0; flow rate of 0.8 ml/min; and detection light wavelength at 220 nm.

Provenance & Evidence