Trisodium Phosphate → Pseudoephedrine

Temperature

30 °C

Microbacterium arborescens IFO 3750 was inoculated to a medium (5 ml) containing 1% glucose, 0.5% peptone, and 0.3% yeast extract, and shake-culturing was carried out at 30° C. for 48 hours. After the cultured solution was centrifuged to give bacterial cells, the cells were placed into a test tube. To this was added 0.1 M sodium phosphate buffer (pH 7.0, 1 ml) and suspended. To this was added dl-2-methylamino-1-phenyl-1-propanone hydrochloride (1 mg) and reaction was allowed to take place under shaking at 30° C. for 24 hours. After the reaction, the reaction solution was centrifuged to remove the bacterial cells and the supernatant was subjected to HPLC to give optically active pseudoephedrine: μ Bondapakphenyl manufactured by Waters Inc.; diameter of 4 mm; length of 300 mm; eluent-0.05 M sodium phosphate buffer (containing 7% acetonitrile); pH 5.0; flow rate of 0.8 ml/min; and detection light wavelength at 220 nm.

1. extractionextract
2. customto give bacterial cells
3. customthe cells were placed into a test tube
4. additionTo this was added dl-
5. customAfter the reaction
6. customto remove the bacterial cells

ORD record

ord-cc316be7e5984aec8b65d05f1b0a3c26

Source dataset

uspto-grants-2004_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06835559B2