Ammonium fumarate → L-Aspartic Acid

HRID 2308929Notes

Reaction equation

HRID 2308929 reaction equation
[C:1]([O-:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([O-:6])=[O:5].[NH4+:9].[NH4+]>[Cl-].[Mg+2].[Cl-]>[NH2:9][C@H:2]([C:1]([OH:8])=[O:7])[CH2:3][C:4]([OH:6])=[O:5]

Participants

3

Reactant 1

HCID 9793847Ammonium fumarate

Product 1

HCID 5960L-Aspartic Acid

Reagents, catalysts & solvents 1

HCID 5360315Magnesium ChlorideSolvent

Procedure

The immobilized Escherichia coli prepared in the above (1) (61 g) was packed in a jacketed column (4 cm×8 cm) and incubated at 37° C. for 48 hours to activate the immobilized cells (aspartase activity: 1980 m moles/hr). After activation, 1 M ammonium fumarate solution containing 1 mM magnesium chloride (pH 8.5, 1,000 ml) was passed through the column at 37° C. at the flow rate of 50 ml/hr. The effluent was adjusted to pH 2.8 to precipitate crystals and the crystals were filtered off to give L-aspartic acid (128 g).

Workup

  1. customwas passed through the column at 37° C. at the flow rate of 50 ml/hr
  2. customto precipitate crystals
  3. filtrationthe crystals were filtered off

Sources & evidence

ORD record
ord-447ee708fb6f48b68df1ce31aad9dabb
Source dataset
uspto-grants-1987_09
Patent number
US04692409

Reaction notes

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