反应详情

温度

37 °C

To a 10 mL potassium phosphate buffer (0.1M, pH 7.5) were added 10 mM DTT, 0.5M pyruvate, 0.1M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3-) resin, eluted with zero to 1M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.

1. customThe reaction mixture was chromatographed with a Dowex-1 (HCO3-) resin
2. washeluted with zero to 1M ammonium bicarbonate gradient
3. additionThe fractions containing KDN
4. customto remove the volatile salt
5. customto give a 78 percent yield of KDN