Acetic Acid → 4-Nitroaniline

HRID 2351833Notes

Reaction equation

HRID 2351833 reaction equation
C1[C@@H](C(NCC(N[C@H](C([NH:24][C:25]2[CH:30]=[CH:29][C:28]([N+:31]([O-:33])=[O:32])=[CH:27][CH:26]=2)=O)CCCN=C(N)N)=O)=O)NC(=O)C1.C(O)(=O)C>C(O)C(N)(CO)CO.Cl>[N+:31]([C:28]1[CH:29]=[CH:30][C:25]([NH2:24])=[CH:26][CH:27]=1)([O-:33])=[O:32]

Participants

4

Reactant 2

HCID 176Acetic AcidHCID 121898N-(2-{[5-Carbamimidamido-1-(4-nitroanilino)-1-oxopentan-2-yl]imino}-2-hydroxyethyl)-5-hydroxy-3,4-dihydro-2H-pyrrole-2-carboximidic acid

Product 1

HCID 74754-Nitroaniline

Reagents, catalysts & solvents 1

HCID 93573Tromethamine hydrochlorideSolvent

Procedure

An inhibitor sample is dissolved in a 0.05 M Tris-hydrochloric acid buffer solution (pH=8.8) to make the total volume to 400 μl. To this solution, 50 μl of a 1 mM S-2444 solution is added and the mixture is incubated at a temperature of 37° C. for 5 minutes in a constant temperature bath. Then, 50 μl of a 500 unit/ml human urokinase is added and the mixture is incubated at a temperature of 37° C. for 5 minutes. Thereafter, 50 μl of 50% acetic acid is added to terminate the reaction. The absorbance of the p-nitroaniline formed in the reaction mixture is measured at 405 nm. Thus, the concentration I50 (μmol) of the inhibitor sample, at which the absorbance is one half (i.e., 1/2) of that obtained in the absence of the inhibitor sample, is determined.

Workup

  1. waitthe mixture is incubated at a temperature of 37° C. for 5 minutes
  2. customto terminate
  3. customthe reaction

Sources & evidence

ORD record
ord-1a059af726754eaea2def3b46cd34e04
Source dataset
uspto-grants-1989_10
Patent number
US04873253

Reaction notes

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