Phenolphthalein → Ethyl 2-(2-aminothiazole-4-yl)-2-hydroxyiminoacetate

Phenolphthalein indicator (3 drops) was added to a solution of hydroxylamine hydrochloride (4.2 g.) in dry methanol (60 ml.). To the solution was dropwise added with stirring at ambient temperature 1N methanol solution of sodium methoxide (60 ml.) until the color of the solution was changed to purplish red. Hydroxylamine hydrochloride was added thereto by small portions until the solution was changed to colorless solution. The mixture was stirred for 30 minutes at ambient temperature. After precipitating sodium chloride was filtered off, 2-(2-mesylamino-1,3-thiazol-4-yl)glyoxylic acid (12.5 g.) was added to the filtrate and the mixture was refluxed with stirring for 1.5 hours. The reaction mixture was cooled to precipitate crystals. The crystals were collected by filtration and dried to give crude 2-hydroxyimino-2-(2-mesylamino-1,3-thiazol-4-yl)acetic acid (a mixture of syn and anti isomers) (5.5 g.). The filtrate was concentrated to the volume of 1/4 and ether was added thereto. Precipitating crystals were collected by filtration, washed with ether and dried to give the same compound (8.78 g.). Total yield (14.3 g.). (3) A mixture of ethyl 2-hydroxyimino-4-bromoacetoacetate (a mixture of syn and anti isomers) (2.4 g) and thiourea (0.76 g) in ethanol (15 ml) was stirred for 1 hour at 60° C. Ethanol was distilled off under reduced pressure and water was added to the residue. The resultant mixture was adjusted to pH 1.0 and washed with ethyl acetate. The aqueous layer was adjusted to pH 4.5 with triethylamine and extracted with ethyl acetate. The extract was washed with water and a saturated sodium chloride aqueous solution and dried over magnesium sulfate. The solvent was distilled off under reduced pressure and the residue was subjected to column chromatography on silica gel using a mixture of ethyl acetate and benzene (1:3) as developing solvent. The eluates containing syn isomer were collected and concentrated to give ethyl 2-hydroxyimino-2-(2-amino-1,3-thiazol-4-yl)acetate (syn isomer) (0.3 g).

1. additionTo the solution was dropwise added
2. customAfter precipitating sodium chloride
3. filtrationwas filtered off
4. addition2-(2-mesylamino-1,3-thiazol-4-yl)glyoxylic acid (12.5 g.) was added to the filtrate
5. temperaturethe mixture was refluxed
6. stirringwith stirring for 1.5 hours
7. temperatureThe reaction mixture was cooled
8. customto precipitate crystals
9. filtrationThe crystals were collected by filtration
10. customdried
11. customto give crude 2-hydroxyimino-2-(2-mesylamino-1,3-thiazol-4-yl)acetic acid (
12. additiona mixture of syn and anti isomers) (5.5 g.)
13. concentrationThe filtrate was concentrated to the volume of 1/4 and ether
14. additionwas added
15. customPrecipitating crystals
16. filtrationwere collected by filtration
17. washwashed with ether
18. customdried
19. customto give the same compound (8.78 g.)
20. customTotal yield (14.3 g.)
21. stirringwas stirred for 1 hour at 60° C
22. distillationEthanol was distilled off under reduced pressure and water
23. additionwas added to the residue
24. washwashed with ethyl acetate
25. extractionextracted with ethyl acetate
26. washThe extract was washed with water
27. dry with materiala saturated sodium chloride aqueous solution and dried over magnesium sulfate
28. distillationThe solvent was distilled off under reduced pressure
29. additiona mixture of ethyl acetate and benzene (1:3)
30. additionThe eluates containing syn isomer
31. customwere collected
32. concentrationconcentrated

ORD record

ord-5fa8c50d076149f490f9e9d9f9fa3dbe

Source dataset

uspto-grants-1989_10

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04877873