Imidazole → HCID 178419078, compound

Participants

Reaction Steps

Buffer C: 20 mM Tris-HCL (pH˜8.5), 100 mM KCl, 100 mM imidazole, 10 mM 2-mercaptoethanol, 10% (v/v) glycerol at 4° C., for example, 1 L (or 200 mL): 9 (1.8) mL 1 M Tris-HCl, 11 (2.2) mL 1 M Tris base, 50 (10 mL 2 M KCl, 100 (20) mL 1 M imidazole, 0.70 (0.14) mL 14.3 M 2-mercaptoethanol (add just before using), 100 (20) mL 100% glycerol (molecular biology grade), 729.30 (145.86) mL water.

Provenance & Evidence