Imidazole → HCID 178419078, compound

HRID 2353997Notes

Reaction equation

HRID 2353997 reaction equation
C(O)[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3]O.[Cl-].[K+].[NH:11]1[CH:15]=[CH:14][N:13]=[CH:12]1.SCC[OH:19].C(O)C(N)(CO)CO.Cl>O.OCC(CO)O>[NH2:7][C@H:2]([C:5]([OH:19])=[O:6])[CH2:3][C:14]1[N:13]=[CH:12][NH:11][CH:15]=1

Participants

8

Reactant 5

HCID 795Imidazole2×HCID 15672-Mercaptoethanol2×HCID 4873Potassium Chloride2×HCID 6503Tris(hydroxymethyl)aminomethane2×HCID 93573Tromethamine hydrochloride

Product 1

HCID 178419078HCID 178419078, compound

Reagents, catalysts & solvents 2

HCID 753GlycerolSolvent2×HCID 962WaterSolvent

Procedure

Buffer C: 20 mM Tris-HCL (pH˜8.5), 100 mM KCl, 100 mM imidazole, 10 mM 2-mercaptoethanol, 10% (v/v) glycerol at 4° C., for example, 1 L (or 200 mL): 9 (1.8) mL 1 M Tris-HCl, 11 (2.2) mL 1 M Tris base, 50 (10 mL 2 M KCl, 100 (20) mL 1 M imidazole, 0.70 (0.14) mL 14.3 M 2-mercaptoethanol (add just before using), 100 (20) mL 100% glycerol (molecular biology grade), 729.30 (145.86) mL water.

Workup

  1. customat 4° C.

Sources & evidence

ORD record
ord-c91633a81eda41319f900290f54f2a64
Source dataset
uspto-grants-2010_07
Patent number
US07750135B2

Reaction notes

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