反应详情

Combine (S)-2-[N-(t-butoxycarbonyl)-allylamino-3-phenyl-propionic acid methyl ester (0.32 g, 1.0 mmol) and 1M sodium hydroxide (10 mL, 10 mmol) in ethanol (10 mL). Stir for 4 hours. Acidify the reaction mixture with 1M hydrochloric acid and extract with ethyl acetate. Separate the layers, dry the organic layer over MgSO4, filter, and evaporate in vacuo. Chromatograph on silica gel eluting with 3% methanol/dichloromethane to give the title compound: TLC Rf =0.40 (silica gel, 5% methanol/dichloromethane).

1. extractionextract with ethyl acetate
2. customSeparate the layers
3. dry with materialdry the organic layer over MgSO4
4. filtrationfilter
5. customevaporate in vacuo