Water → Theophylline

HRID 2371353Notes

Reaction equation

HRID 2371353 reaction equation
C([C:4]1[NH:16][C:15]2[C:14](=[O:17])[N:9]([CH2:10]C(O)=O)[C:8](=[O:18])[N:7]([CH3:19])[C:6]=2[N:5]=1)CC.ON1C(=O)CCC1=O.O>CN(C)C=O>[N:9]1([C:14](=[O:17])[C:15]2[NH:16][CH:4]=[N:5][C:6]=2[N:7]([CH3:19])[C:8]1=[O:18])[CH3:10]

Participants

5

Reactant 3

HCID 962WaterHCID 80170N-HydroxysuccinimideHCID 86745509HCID 86745509, compound

Product 1

HCID 2153Theophylline

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent

Procedure

5 mg of 8-propylcarboxytheophylline dissolved in 100 μl of dimethylformamide (DMF) was mixed with 3 mg of N-hydroxysuccinimide and 5 mg of water-soluble carbodiimide, and stirred at room temperature for 2 hours to obtain activated theophylline. 500 μl of the above activated theophylline solution was added to 10 mg of horse ferritin dissolved in 1 ml of 0.1M sodium hydrogen carbonate aqueous solution, and allowed to stand at room themperature for 1 hour. Unreacted substances were removed by using a Sephadex G-25 gel column previously equilibrated with a phosphate-buffered saline solution of pH 7.0 to obtain 9 mg of the object macromolecular antigen of horse ferritin-theophylline conjugate.

Sources & evidence

ORD record
ord-d76d97f2113e44b79ae591031643b570
Source dataset
uspto-grants-1992_03
Patent number
US05093081

Reaction notes

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