反应详情

温度

30 °C

Propionibacterium freudenreichii IFO12425 was inoculated into 10 liters of a culture medium (pH: 6.5) containing 0.35% of glucose, 0.30% of peptone, 0.30% of yeast extract, and 0.05% of KH2PO4. This bacteria was cultured at 30° C. using a jar fermenter; 20 g of wet microorganisms were collected by centrifugation and immersed in acetone for 2 hours. Separately, 30 liters of tris-HCl solution prepared in the same manner as in Example 1 was placed in a 70-liter reaction vessel. To the tris-HCl solution was added 20 g of the acetone-treated microorganism body. The resulting solution was maintained at 30° C., and 20 liters of acetyl phosphate prepared as in the Reference Example was added in 5-liter portions. The reaction solution yielded 3.6 kg of dicalcium fructose-1,6-diphosphate salt following treatment with calcium chloride in the same manner as described in Example 1.

1. extractionextract
2. customwas cultured at 30° C.
3. custom20 g of wet microorganisms were collected by centrifugation
4. customSeparately, 30 liters of tris-HCl solution prepared in the same manner as in Example 1
5. customwas placed in a 70-liter reaction vessel
6. additiontreated microorganism body
7. additionwas added in 5-liter portions