(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-Fructose-1,6-diphosphate dicalcium salt

Participants

Reaction Conditions

Reaction Steps

Propionibacterium freudenreichii IFO12425 was inoculated into 10 liters of a culture medium (pH: 6.5) containing 0.35% of glucose, 0.30% of peptone, 0.30% of yeast extract, and 0.05% of KH2PO4. This bacteria was cultured at 30° C. using a jar fermenter; 20 g of wet microorganisms were collected by centrifugation and immersed in acetone for 2 hours. Separately, 30 liters of tris-HCl solution prepared in the same manner as in Example 1 was placed in a 70-liter reaction vessel. To the tris-HCl solution was added 20 g of the acetone-treated microorganism body. The resulting solution was maintained at 30° C., and 20 liters of acetyl phosphate prepared as in the Reference Example was added in 5-liter portions. The reaction solution yielded 3.6 kg of dicalcium fructose-1,6-diphosphate salt following treatment with calcium chloride in the same manner as described in Example 1.

Provenance & Evidence