反应详情

An anion-exchange resin column 5 cm in diameter and 50 cm in length (Dowex SAR, made by Muromachi Kagaku Kogyo K. K.) was brought to equilibrium using an aqueous solution containing 0.04M sodium chloride and 0.01N hydrochloric acid. The above-mentioned 6.6 liters of fructose-1,6-diphosphate solution was put on the column. The column was then washed with about 30 liters of the equilibrating solution. Thereafter, the fructose-1,6-diphosphate was eluted using 20 liters of an aqueous solution containing 0.4M sodium chloride and 0.01N hydrochloric acid, resulting in 20 liters of an aqueous 26 mM fructose-1,6-diphosphate solution free from phosphate. Sodium chloride was eliminated from this aqueous fructose-1,6-diphosphate solution by means of an electrodialysis apparatus (Model G-3, dialysis membrane AC-110-800 made by Asahi Kasei Kogyo K. K.). The dialyzed solution was concentrated by rotary evaporation and freeze-dried, to give 232 g of powdery sodium fructose-1,6-diphosphate.

1. washThe column was then washed with about 30 liters of the equilibrating solution
2. washThereafter, the fructose-1,6-diphosphate was eluted
3. additioncontaining 0.4M sodium chloride and 0.01N hydrochloric acid