HRID2374129

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：C[S+](CC[C@H](N)C(=O)O)C[C@H]1O[C@@H](n2cnc3c(N)ncnc32)[C@H](O)[C@@H]1O

HCID34756

5'-[[(3S)-3-Amino-3-carboxypropyl]methylsulfonio]-5'-deoxy-adenosine

C15H23N6O5S+

HCID443902

未命名化合物

HCID5280373

Biochanin A

C16H12O5

结构：COc1ccc(C2C(=O)c3c(O)cc(O)cc3OC2O)cc1

HCID9904478

未命名化合物

PRODUCTS

生成物

HCID13257279

2-Hydroxy-3-phenyl-2,3-dihydro-4H-1-benzopyran-4-one

C15H12O3

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID8019

2-Methoxyethanol

C3H8O2

PROCEDURE

实验过程

The assay of 2,5,7-trihydroxy-4′-methoxyisoflavanone dehyderatase (biochanin A synthetase) was carried out as follows. 2,5,7,4′-tetrahydroxyisoflavanone (10 nmol) dissolved in 2-methoxyethanol was incubated together with licorice HI4′OMT (1 μg) and 1 μmol of S-adenosyl-L-methionine (SAM) at 30° C. for 15 minutes.