HuagongsheHuagongshe Chemistry Print Report · For research reference only
S-adenosyl-L-methionine → 2-Hydroxy-3-phenyl-2,3-dihydro-4H-1-benzopyran-4-one
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | S-adenosyl-L-methionine | HCID 34756 | — |
| Reactant | 2-Hydroxy-2,3-dihydrogenistein | HCID 443902 | — |
| Reactant | Biochanin A | HCID 5280373 | — |
| Reactant | 2,5,7-Trihydroxy-4'-methoxyisoflavanon | HCID 9904478 | — |
| Solvent | 2-Methoxyethanol | HCID 8019 | — |
| Product | 2-Hydroxy-3-phenyl-2,3-dihydro-4H-1-benzopyran-4-one | HCID 13257279 | — |
Reaction Steps
The assay of 2,5,7-trihydroxy-4′-methoxyisoflavanone dehyderatase (biochanin A synthetase) was carried out as follows. 2,5,7,4′-tetrahydroxyisoflavanone (10 nmol) dissolved in 2-methoxyethanol was incubated together with licorice HI4′OMT (1 μg) and 1 μmol of S-adenosyl-L-methionine (SAM) at 30° C. for 15 minutes.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07462478B2 |
| Source dataset | uspto-grants-2008_12 |
| ORD ID | ord-14c72a6ee09a4930bc514b9742a2ff9c |
| Source note | uspto-grants-2008_12 |