beta-Maltose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

Ten g of maltose was suspended in 8.5 ml of 50 mM acetate buffer (pH 6.0), and 900 units of pullulanase derived from microorganisms of the genus Klebsiella (produced by Amano Pharmaceutical Co., Japan) were added thereto and reacted for 48 hours at 60° C. Regarding the enzyme activity of pullulanase, one unit thereof indicates an amount producing a reducing sugar corresponding to 1 μmol of glucose in one minute when reacted with a pullulan at a pH of 6.0 and a temperature of 40° C.

Provenance & Evidence