beta-Maltose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 2380185Notes

Reaction equation

HRID 2380185 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]>C([O-])(=O)C>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:23])[OH:7])[OH:22])[OH:21])[OH:20]

Participants

3

Reactant 1

HCID 6255beta-Maltose

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Reagents, catalysts & solvents 1

HCID 175AcetateSolvent

Procedure

Ten g of maltose was suspended in 8.5 ml of 50 mM acetate buffer (pH 6.0), and 900 units of pullulanase derived from microorganisms of the genus Klebsiella (produced by Amano Pharmaceutical Co., Japan) were added thereto and reacted for 48 hours at 60° C. Regarding the enzyme activity of pullulanase, one unit thereof indicates an amount producing a reducing sugar corresponding to 1 μmol of glucose in one minute when reacted with a pullulan at a pH of 6.0 and a temperature of 40° C.

Workup

  1. custom900 units of pullulanase derived from microorganisms of the genus Klebsiella (produced by Amano Pharmaceutical Co., Japan)
  2. additionwere added
  3. customreacted for 48 hours at 60° C

Sources & evidence

ORD record
ord-78563c262e1948fbad1f78a5b8346446
Source dataset
uspto-grants-1994_11
Patent number
US05366879

Reaction notes

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