反应详情

1-tert-butyl-3-(3-nitrophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (23 mg, 0.055 mmol) was dissolved in a solution of formic acid (5 mL) and conc. HCl (0.1 mL) and heated to reflux for 2 hours. Reaction was concentrated in vacuo and purified by RP-HPLC (MeCN:H2O:0.1% TFA). ESI-MS (M+H)+ m/z calcd 257.1, found 257.3.

1. temperatureheated
2. temperatureto reflux for 2 hours
3. customReaction
4. concentrationwas concentrated in vacuo
5. custompurified by RP-HPLC (MeCN:H2O:0.1% TFA)