Phosphoric Acid → HCID 59471170, compound

HRID 2384547Notes

Reaction equation

HRID 2384547 reaction equation
[O:1]=[C:2]1[CH2:6][CH2:5][CH2:4][CH:3]1[CH2:7][CH:8]([NH:12]C(N)=O)[C:9]([OH:11])=[O:10].[OH-].[Na+].OP(O)(O)=O>O>[NH2:12][C@@H:8]([CH2:7][CH:3]1[CH2:4][CH2:5][CH2:6][C:2]1=[O:1])[C:9]([OH:11])=[O:10]

Participants

5

Reactant 3

HCID 1004Phosphoric AcidHCID 14798Sodium HydroxideHCID 25262532HCID 25262532, compound

Product 1

HCID 59471170HCID 59471170, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
28 °C

Procedure

3-(2-oxocyclopentyl)-2-ureidopropanoic acid (15.0 g, 0.07 mol) was suspended in water (90 mL) and the pH was adjusted to 7.3 with a 32% NaOH solution (8.30 g). Then, MnCl2 solution (3.75 mL, 500 mmol/L) was added and 60 g of wet cell slurry obtained according to ‘Expression of Hyu genes in Escherichia coli RV308’ (see above) was added. The reaction was stirred at 28° C. for 20 h and the pH was decreased to 4 or 6, depending on the conditions as used in Example 4, with 85% H3PO4. The reaction mixture was then centrifuged (12.000 rpm) and the clear liquid was separated from the remaining cell mass.

Workup

  1. custom60 g of wet cell slurry obtained
  2. addition’ (see above) was added
  3. customthe clear liquid was separated from the remaining cell mass

Sources & evidence

ORD record
ord-5ae625a490fb416fb1e245e2282bd220
Source dataset
uspto-grants-2012_09
Patent number
US08263784B2

Reaction notes

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