Phosphoric Acid → HCID 59471170, compound

Participants

Reaction Conditions

Reaction Steps

3-(2-oxocyclopentyl)-2-ureidopropanoic acid (15.0 g, 0.07 mol) was suspended in water (90 mL) and the pH was adjusted to 7.3 with a 32% NaOH solution (8.30 g). Then, MnCl2 solution (3.75 mL, 500 mmol/L) was added and 60 g of wet cell slurry obtained according to ‘Expression of Hyu genes in Escherichia coli RV308’ (see above) was added. The reaction was stirred at 28° C. for 20 h and the pH was decreased to 4 or 6, depending on the conditions as used in Example 4, with 85% H3PO4. The reaction mixture was then centrifuged (12.000 rpm) and the clear liquid was separated from the remaining cell mass.

Provenance & Evidence