Urea → Bis(eta-cyclopentadienyl) iron

Participants

Reaction Steps

Urea (810 mg), a water-soluble carbodiimide (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; 100 mg) and ferrocenecarboxylic acid (80 mg) were subjected to sonication in a 0.15M HEPES buffer (4 ml, pH 7.3), and the mixture subjected to sonication was again adjusted to a pH value of 7.2 to 7.3. the ferrocenecarboxylic acid was saturated at this pH value. The GOD hydrazide solution (2 ml) prepared in the above (b) was added thereto, the pH value was confirmed and adjusted according to need, and the reaction mixture was allowed to stand at 0° C. in a dark place overnight. The reaction mixture was dialyzed by using a 0.1M phosphate buffer (pH 6.0) as an external solution. In the dialysis, the external solution was replaced four times with fresh external solution to remove excess reagents, thereby providing GOD having ferrocene introduced into its body and side chain.

Provenance & Evidence