Urea → Bis(eta-cyclopentadienyl) iron

HRID 2392963Notes

Reaction equation

HRID 2392963 reaction equation
NC(N)=O.[C-:5]1(C(O)=O)[CH:9]=[CH:8][CH:7]=[CH:6]1.[CH-:13]1[CH:17]=[CH:16][CH:15]=[CH:14]1.[Fe+2:18].C1N(CCO)CCN(CCS(O)(=O)=O)C1>O>[CH-:5]1[CH:9]=[CH:8][CH:7]=[CH:6]1.[CH-:13]1[CH:17]=[CH:16][CH:15]=[CH:14]1.[Fe+2:18]

Participants

5

Reactant 3

HCID 1176UreaHCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 157642301271-42-72×

Product 1

HCID 7611Bis(eta-cyclopentadienyl) iron

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Urea (810 mg), a water-soluble carbodiimide (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; 100 mg) and ferrocenecarboxylic acid (80 mg) were subjected to sonication in a 0.15M HEPES buffer (4 ml, pH 7.3), and the mixture subjected to sonication was again adjusted to a pH value of 7.2 to 7.3. the ferrocenecarboxylic acid was saturated at this pH value. The GOD hydrazide solution (2 ml) prepared in the above (b) was added thereto, the pH value was confirmed and adjusted according to need, and the reaction mixture was allowed to stand at 0° C. in a dark place overnight. The reaction mixture was dialyzed by using a 0.1M phosphate buffer (pH 6.0) as an external solution. In the dialysis, the external solution was replaced four times with fresh external solution to remove excess reagents, thereby providing GOD having ferrocene introduced into its body and side chain.

Workup

  1. customthe mixture subjected to sonication
  2. additionwas added
  3. customto stand at 0° C. in a dark place overnight
  4. customThe reaction mixture was dialyzed
  5. customIn the dialysis
  6. customto remove excess reagents
  7. additionintroduced into its body and side chain

Sources & evidence

ORD record
ord-7d59e22b2c15433ebea6e27a955b863f
Source dataset
uspto-grants-1998_09
Patent number
US05804047

Reaction notes

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