Urea → Bis(eta-cyclopentadienyl) iron

Participants

Reaction Steps

Urea (4050 mg), (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (500 mg) and ferrocenecarboxylic acid (450 mg) were dissolved in a 0.15M HEPES buffer (25 ml). The solution was adjusted to the pH value of 7.3. The GOD solution was added thereto, and the mixture was allowed to stand at 4° C. in a dark place overnight while confirming several times that the pH value was 7.3. The reaction mixture was dialyzed by using a 0.1M phosphate buffer (pH 6.0) as external solution. In the dialysis, the external solution was replaced four times with fresh external solution to remove excess reagents, thereby providing GOD having ferrocene introduced into its body.

Provenance & Evidence