Urea → Bis(eta-cyclopentadienyl) iron

HRID 2392965Notes

Reaction equation

HRID 2392965 reaction equation
NC(N)=O.Cl.C(N=C=NCCCN(C)C)C.[C-:17]1(C(O)=O)[CH:21]=[CH:20][CH:19]=[CH:18]1.[CH-:25]1[CH:29]=[CH:28][CH:27]=[CH:26]1.[Fe+2:30]>C1N(CCO)CCN(CCS(O)(=O)=O)C1>[CH-:17]1[CH:21]=[CH:20][CH:19]=[CH:18]1.[CH-:25]1[CH:29]=[CH:28][CH:27]=[CH:26]1.[Fe+2:30]

Participants

5

Reactant 3

HCID 1176UreaHCID 27239391,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochlorideHCID 157642301271-42-7

Product 1

HCID 7611Bis(eta-cyclopentadienyl) iron

Reagents, catalysts & solvents 1

HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidSolvent

Procedure

Urea (4050 mg), (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (500 mg) and ferrocenecarboxylic acid (450 mg) were dissolved in a 0.15M HEPES buffer (25 ml). The solution was adjusted to the pH value of 7.3. The GOD solution was added thereto, and the mixture was allowed to stand at 4° C. in a dark place overnight while confirming several times that the pH value was 7.3. The reaction mixture was dialyzed by using a 0.1M phosphate buffer (pH 6.0) as external solution. In the dialysis, the external solution was replaced four times with fresh external solution to remove excess reagents, thereby providing GOD having ferrocene introduced into its body.

Workup

  1. additionThe GOD solution was added
  2. customto stand at 4° C. in a dark place overnight
  3. customThe reaction mixture was dialyzed
  4. customIn the dialysis
  5. customto remove excess reagents
  6. additionintroduced into its body

Sources & evidence

ORD record
ord-ab15046c89014f8f96aa50364361ff84
Source dataset
uspto-grants-1998_09
Patent number
US05804047

Reaction notes

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