Tromethamine hydrochloride → 7-Amino-4-methylcoumarin

HRID 2392985Notes

Reaction equation

HRID 2392985 reaction equation
C(O)C(N)(CO)CO.Cl.[CH3:10][C:11]1[C:17]2[CH:18]=[CH:19][C:20]([NH:22]C([C@@H](NC([C@@H](NC([C@@H](NC(OC(C)(C)C)=O)CC3C=CC=CC=3)=O)CO)=O)CCCN=C(N)N)=O)=[CH:21][C:16]=2[O:15][C:13](=[O:14])[CH:12]=1>C(O)(=O)C>[NH2:22][C:20]1[CH:21]=[C:16]2[C:17]([C:11]([CH3:10])=[CH:12][C:13](=[O:14])[O:15]2)=[CH:18][CH:19]=1

Participants

4

Reactant 2

HCID 93573Tromethamine hydrochlorideHCID 1263776-Benzyl-12-(3-carbamimidamidopropyl)-4,7,10-trihydroxy-9-(hydroxymethyl)-2,2-dimethyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-3-oxa-5,8,11-triazatrideca-4,7,10-trien-13-imidic acid

Product 1

HCID 922497-Amino-4-methylcoumarin

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

50 μl of the solution of the trypsin-like protease obtained in Example 1 was added to 1.5 ml of 0.1M Tris-HCl buffer (pH 8.6) containing 100 μM of a synthetic substrate for trypsin Boc-Phe-Ser-Arg-MCA (MCA=methylcoumarinamide), and the mixture was subjected to incubation at 37° C. for 1 hour. 1 ml of 30% acetic acid was then added, the amount of 7-amino-4-methylcoumarin (AMC) formed was determined by fluorescent assay (fluorescence 440 nm, excitating light 380 nm), and the activity of the enzyme was calculated based thereon. An activity of forming 1 pM of AMC in 1 minute is defined as 1 unit (1 unit=1 pM AMC/min).

Sources & evidence

ORD record
ord-90e7b7ab1c4a48bf8edba7da2d586f6f
Source dataset
uspto-grants-1998_09
Patent number
US05804410

Reaction notes

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