Cytidine → N4-Acetylcytidine

Participants

Reaction Steps

Cytidine (1) (7.3 g, 30 mmoles) was suspended in anhydrous N,N-dimethylformamide (120 ml) and to it was added acetic anhydride (3.12 ml, 33 mmoles). The mixture was stirred at room temperature overnight. After removal of the DMF under reduced pressure, the resulting residue was triturated with excess of diethylether (~30 ml) and the crystalline product obtained collected by filtration, washed thoroughly with diethylether and air dried to get a quantitative yield of 2. A small portion was crystallized from ethanol/water mixture (1:1) to obtain crystals for analytical purposes.

Provenance & Evidence