反应详情

In 50 ml of methanol, 4.4 g of methyl N-benzoylphenylalanyltyrosinate was dissolved. After addition of 23 ml of 1 N sodium hydroxide, the solution was stirred for 1 hour. The reaction mixture was neutralized with hydrochloric acid, concentrated and extracted with ethyl acetate. The extract was washed with water, dried and concentrated to dryness. The residue was crystallized from ethyl acetate to give 3.8 g of N-benzoylphenylalanyltyrosine with a mp. 191°-192° C., in a yield of 87%. Analysis, calculated for C25H24O5N2 : C, 69.43; H, 5.59; N, 6.48; found C, 68.98; H, 5.72; N, 6.37.

1. concentrationconcentrated
2. extractionextracted with ethyl acetate
3. washThe extract was washed with water
4. customdried
5. concentrationconcentrated to dryness
6. customThe residue was crystallized from ethyl acetate