Sodium Carbonate → HCID 91746173, compound

Participants

Reaction Steps

10 g of soybean protein (ESUSAN-MEAT; trade name; AJINOMOTO Inc. ), 1 g of dipotassium hydrogen phosphate and 0.2 g of magnesium sulfate 7hydrates were dissolved in 500 ml of refined water, and 50 g of cholic acid, 5 g of sodium hydroxide and 2 g of sodium carbonate were dissolved in 500 ml of refined water, then both solutions were sterilized at the temperature of 121° C. for 15 minutes. After cooling, two solutions were mixed and the mixture was used for a culture medium (pH10.2). Thereafter, the same procedure as Example 1 was proceeded and 12-keto-cholic acid was obtained. The ratio in the product was cholic acid 0.5% and 12-keto-cholic acid 99.5%.

Provenance & Evidence