Sodium Carbonate → HCID 91746173, compound

HRID 2399664Notes

Reaction equation

HRID 2399664 reaction equation
P([O-])([O-])(O)=O.[K+].[K+].S([O-])([O-])(=O)=O.[Mg+2].[CH3:14][C@@H:15]([C@@H:21]1[C@@:25]2([CH3:42])[C@@H:26]([OH:41])[CH2:27][C@@H:28]3[C@@:33]4([CH3:39])[CH2:34][CH2:35][C@@H:36]([OH:38])[CH2:37][C@H:32]4[CH2:31][C@@H:30]([OH:40])[C@H:29]3[C@@H:24]2[CH2:23][CH2:22]1)[CH2:16][CH2:17][C:18]([OH:20])=[O:19].[OH-].[Na+].C(=O)([O-])[O-].[Na+].[Na+]>O>[CH3:14][C@@H:15]([C@@H:21]1[C@@:25]2([CH3:42])[C:26]([CH2:27][CH:28]3[C@@:33]4([CH3:39])[CH2:34][CH2:35][C@@H:36]([OH:38])[CH2:37][C@H:32]4[CH2:31][C@@H:30]([OH:40])[C@H:29]3[C@@H:24]2[CH2:23][CH2:22]1)=[O:41])[CH2:16][CH2:17][C:18]([OH:20])=[O:19]

Participants

7

Reactant 5

HCID 10340Sodium CarbonateHCID 14798Sodium HydroxideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24450Potassium Phosphate, DibasicHCID 221493Cholic Acid

Product 1

HCID 91746173HCID 91746173, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

10 g of soybean protein (ESUSAN-MEAT; trade name; AJINOMOTO Inc. ), 1 g of dipotassium hydrogen phosphate and 0.2 g of magnesium sulfate 7hydrates were dissolved in 500 ml of refined water, and 50 g of cholic acid, 5 g of sodium hydroxide and 2 g of sodium carbonate were dissolved in 500 ml of refined water, then both solutions were sterilized at the temperature of 121° C. for 15 minutes. After cooling, two solutions were mixed and the mixture was used for a culture medium (pH10.2). Thereafter, the same procedure as Example 1 was proceeded and 12-keto-cholic acid was obtained. The ratio in the product was cholic acid 0.5% and 12-keto-cholic acid 99.5%.

Workup

  1. temperatureAfter cooling
  2. additiontwo solutions were mixed

Sources & evidence

ORD record
ord-2e5b81a105ee4d438fcaef862261e2a7
Source dataset
uspto-grants-1995_09
Patent number
US05451510

Reaction notes

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