Sodium Hydroxide → HCID 91746173, compound

HRID 2399665Notes

Reaction equation

[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C@@H:13]([OH:28])[CH2:14][C@@H:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][C@@H:17]([OH:27])[C@H:16]3[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([OH:7])=[O:6].[OH-].[Na+]>>[CH3:1][C@@H:2]([C@@H:8]1[C@@:12]2([CH3:29])[C:13]([CH2:14][CH:15]3[C@@:20]4([CH3:26])[CH2:21][CH2:22][C@@H:23]([OH:25])[CH2:24][C@H:19]4[CH2:18][C@@H:17]([OH:27])[C@H:16]3[C@@H:11]2[CH2:10][CH2:9]1)=[O:28])[CH2:3][CH2:4][C:5]([OH:7])=[O:6]

Participants

Reactant 2

HCID 14798Sodium Hydroxide

HCID 221493Cholic Acid

Product 1

HCID 91746173HCID 91746173, compound

Conditions

Temperature: 30 °C

Procedure

Bacillus sp. TTUR 2-M4-336 was cultivated under shaking at the temperature of 30° C. for 24 hours in a test tube containing 20 ml of the culture medium of above composition except cholic acid and sodium hydroxide were excluded, thus a bacterium solution was prepared. 20 ml of above culture medium was placed into a test tube(30φ×190 mm), 40 ml of said bacterium solution was aseptically inoculated therein, and the bacterium in the test tube was cultivated under agitation (300 rpm) at the aeration rate 21/min. at 30° C. for three days. A trace amount of antifoaming agent (EINOL; trade name; BIOTT Co. LTD) was added during this operation. And thereafter, the same procedure as Example 23 was proceeded and 12-keto-cholic acid was obtained. The ratio in the product was cholic acid 0.7%, 12-keto-cholic acid 99.3% (recovery rate: 99.2% ).

Workup

customthus a bacterium solution was prepared
waitat 30° C. for three days
additionA trace amount of antifoaming agent (EINOL; trade name; BIOTT Co. LTD) was added during this operation

Sources & evidence

ORD record: ord-eb9dd1a653df4166b15ea26429b64e62
Source dataset: uspto-grants-1995_09
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05451510

Reaction notes

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