Sodium Hydroxide → HCID 91746173, compound

Participants

Reaction Conditions

Reaction Steps

Bacillus sp. TTUR 2-M4-336 was cultivated under shaking at the temperature of 30° C. for 24 hours in a test tube containing 20 ml of the culture medium of above composition except cholic acid and sodium hydroxide were excluded, thus a bacterium solution was prepared. 20 ml of above culture medium was placed into a test tube(30φ×190 mm), 40 ml of said bacterium solution was aseptically inoculated therein, and the bacterium in the test tube was cultivated under agitation (300 rpm) at the aeration rate 21/min. at 30° C. for three days. A trace amount of antifoaming agent (EINOL; trade name; BIOTT Co. LTD) was added during this operation. And thereafter, the same procedure as Example 23 was proceeded and 12-keto-cholic acid was obtained. The ratio in the product was cholic acid 0.7%, 12-keto-cholic acid 99.3% (recovery rate: 99.2% ).

Provenance & Evidence