Acetic Acid → 14806-72-5

HRID 241471Notes

Reaction equation

HRID 241471 reaction equation
[N:1]([CH2:8][CH2:9][OH:10])([CH2:5][CH2:6][OH:7])[CH2:2][CH2:3][OH:4].[C:11]([OH:14])(=[O:13])[CH3:12]>>[C:11]([OH:14])(=[O:13])[CH3:12].[N:1]([CH2:8][CH2:9][OH:10])([CH2:5][CH2:6][OH:7])[CH2:2][CH2:3][OH:4]

Participants

3

Reactant 2

HCID 176Acetic AcidHCID 7618Triethanolamine

Product 1

HCID 15975214806-72-5

Procedure

Polydispersed CA was also fractionated by Sepharose Q FF (1 ml matrix, prepacked) using a range of triethanolamine acetate concentrations at pH 7.4 in the absence of any salt such as NaCl. The triethanolamine acetate buffer was prepared using triethanolamine and adjusting the pH to 7.4 using acetic acid. Polydispersed CA (40 mg; 1 ml) (22.7 kDa; pd 1.34) was loaded on to a Q FF column (1 ml matrix; prepacked). The bound CA was eluted by passing 1 ml of each triethanolamine acetate buffer (300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400 and 1500 mM triethanolamine acetate) through the column at a flow rate of 1 ml/min with a final wash of the column using 200 mM triethanolamine acetate. A small sample of each fraction (20 il) was then buffer exchanged with water using a micro membrane dialysis system, lyophilised and then analysed by native PAGE (20% TBE gel stained with alcian blue).

Workup

  1. concentrationa range of triethanolamine acetate concentrations at pH 7.4 in the absence of any salt such as NaCl
  2. washThe bound CA was eluted
  3. washa final wash of the column
  4. customa micro membrane dialysis system

Sources & evidence

ORD record
ord-ebd4b4c3ec34404ab824485fb10e3a62
Source dataset
uspto-grants-2014_02
Patent number
US08652334B2

Reaction notes

Loading…