Citric Acid → Citrate phosphate

HRID 2417577Notes

Reaction equation

HRID 2417577 reaction equation
[C:1]([OH:13])(=[O:12])[CH2:2][C:3]([CH2:8][C:9]([OH:11])=[O:10])([C:5]([OH:7])=[O:6])[OH:4].[P:14]([O-:18])([O-:17])([O-:16])=[O:15].[Na+].[Na+].[Na+].[OH-].[Na+]>O>[C:1]([O-:13])(=[O:12])[CH2:2][C:3]([CH2:8][C:9]([O-:11])=[O:10])([C:5]([O-:7])=[O:6])[OH:4].[P:14]([O-:18])([O-:17])([O-:16])=[O:15]

Participants

5

Reactant 3

HCID 311Citric AcidHCID 14798Sodium HydroxideHCID 24243Trisodium Phosphate

Product 1

HCID 21226156Citrate phosphate

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

A citrate-phosphate buffer (pH 3.0) was prepared from 39.8 ml of 0.1 M citric acid and 10.2 ml of 0.2 M dibasic sodium phosphate diluted to 100 ml with distilled water. The lyophilized Fraction A from Preparation 1 was dissolved in this buffer solution in a proportion of 20 mg of Fraction A per ml of buffer. To this was added an aliquot of 5 mg of pepsin in 1 ml of distilled water to give a ratio of Fraction A to pepsin of 100 to 1. The solution was maintained for 24 hours at room temperature and then brought to pH 7.4 by the addition of 0.1 M sodium hydroxide. The resulting product was the crude ragweed polypeptide active immunosuppressant fraction.

Workup

  1. customThe lyophilized Fraction A from Preparation 1
  2. dissolutionwas dissolved in this buffer solution in a proportion of 20 mg of Fraction A per ml of buffer
  3. customto give a ratio of Fraction A

Sources & evidence

ORD record
ord-0645d3f4ce454d4ba4ba54b3309b0218
Source dataset
uspto-grants-1984_09
Patent number
US04469677

Reaction notes

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