Methadone → 6-Dimethylamino-4-(4-hydroxyphenyl)-4-phenylheptan-3-one

HRID 2418998Notes

Reaction equation

HRID 2418998 reaction equation
[CH3:1][CH2:2][C:3]([C:5]([C:18]1[CH:19]=[CH:20][CH:21]=[CH:22][CH:23]=1)([C:12]1[CH:13]=[CH:14][CH:15]=[CH:16][CH:17]=1)[CH2:6][CH:7]([N:9]([CH3:11])[CH3:10])[CH3:8])=[O:4].S(=O)(O)[O-:25].C(O)C(N)(CO)CO>>[CH3:10][N:9]([CH3:11])[CH:7]([CH3:8])[CH2:6][C:5]([C:18]1[CH:19]=[CH:20][CH:21]=[CH:22][CH:23]=1)([C:12]1[CH:13]=[CH:14][C:15]([OH:25])=[CH:16][CH:17]=1)[C:3](=[O:4])[CH2:2][CH3:1]

Participants

4

Reactant 3

HCID 4095MethadoneHCID 6503Tris(hydroxymethyl)aminomethaneHCID 104748Bisulfite

Product 1

HCID 30825326-Dimethylamino-4-(4-hydroxyphenyl)-4-phenylheptan-3-one

Procedure

The procedure of Example 5 is repeated using the methadone derivative 6-methylamino-4-phenyl-4-(4-hydroxyphenyl)heptan-3-one except for the following modifications. Instead of mixing the NaI125 reaction mixture on a Vortex mixer the mixture is shaken gently for 90 seconds and after addition of the bisulfite the mixture is shaken gently for 30 seconds. Finally 2.0 ml of 0.1 M Tris buffer pH 7.0 is used prior to the column chromatography step. The resulting above-captioned labeled antigen is obtained in Fractions #22-27 at a radioactive concentration of 10.5μCi/ml. EXAMPLE 7

Workup

  1. additionInstead of mixing
  2. customthe NaI125 reaction mixture on a Vortex mixer the mixture
  3. stirringis shaken gently for 30 seconds
  4. customThe resulting above-captioned labeled antigen is obtained in Fractions #22-27 at a radioactive concentration of 10.5μCi/ml

Sources & evidence

ORD record
ord-dfa4f5ff2bda44c28a12b89f9ce458f7
Source dataset
uspto-grants-1978_08
Patent number
US04104367

Reaction notes

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