Phosphate → D-(-)-Fructose

Participants

Reaction Conditions

Reaction Steps

A given amount of aqueous glucose isomerase solution was added to a mixture of 0.2 ml of a 1 M aqueous D-glucose, 0.2 ml of a 0.05 M MgSO4 ·7H2O and 0.2 ml of a 0.5 M phosphate buffer (pH 7.2) and the resulting mixture was diluted with demineralized water to 2ml. The mixture was maintained at 70° C for 60 minutes to effect isomerization. The reaction was stopped by addition of 2 ml of a 0.5 M aqueous HClO4, and the amount of fructose produced was measured by the cystein-carbazole method. The activity was the amount of fructose produced with 1 ml of the aqueous glucose isomerase solution under the conditions mentioned above and was expressed by the unit "U" which represented the production of 1 mg of fructose.

Provenance & Evidence