Phosphate → D-(-)-Fructose
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[O-]S([O-])(=O)=O.[Mg+2].P([O-])([O-])([O-])=O>O>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]Participants
5
Reactant 3
Product 1
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 70 °C
Procedure
A given amount of aqueous glucose isomerase solution was added to a mixture of 0.2 ml of a 1 M aqueous D-glucose, 0.2 ml of a 0.05 M MgSO4 ·7H2O and 0.2 ml of a 0.5 M phosphate buffer (pH 7.2) and the resulting mixture was diluted with demineralized water to 2ml. The mixture was maintained at 70° C for 60 minutes to effect isomerization. The reaction was stopped by addition of 2 ml of a 0.5 M aqueous HClO4, and the amount of fructose produced was measured by the cystein-carbazole method. The activity was the amount of fructose produced with 1 ml of the aqueous glucose isomerase solution under the conditions mentioned above and was expressed by the unit "U" which represented the production of 1 mg of fructose.
Sources & evidence
- ORD record
- ord-a6c19484347e4bf78c5a2afccd8cdb4f
- Source dataset
- uspto-grants-1978_08
- Patent number
- US04106993
Reaction notes
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