Ethyl Acetate → 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [1-(4-bromo-phenyl)-cyclopropyl]-amide

Beteiligte Stoffe

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Reaktionsschritte

To a stirred solution of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-(2-trimethylsilanylethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [1-(4-bromo-phenyl)-cyclopropyl]-amide (50 mg, 0.078 mmol) in THF (3 mL) was added a solution of TBAF (1.0 M in THF, 1.18 mL, 1.18 mmol) at room temperature. The resulting light brown solution was heated to 85° C. and stirred for 3 h at which time TLC analysis (EA, Rf=0.3) indicated the absence of starting material. Then, the reaction mixture was cooled to room temperature and 3 mL of acetone and 9 mL of saturated sodium bicarbonate solution were added and the resulting suspension was stirred for 1 h. The reaction suspension was diluted with water and the organic compound was extracted into EtOAc (3×25 mL) and the combined extracts were washed with brine solution and dried over MgSO4. Filtration of the drying agent and removal of the solvent under vacuum gave the crude solid which was dissolved in acetonitrile with heating. The resulting greenish solution was stored in the refrigerator for 15 h and the solids were collected by filtration and washed with acetonitrile. After drying in air, 25 mg (63%) of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [1-(4-bromo-phenyl)-cyclopropyl]-amide was isolated as a off-white solid. LC-MS (M+H)+=507; 1H NMR (300 MHz, DMSO-d6) δ: 12.91 (s, 1H), 9.11 (s, 1H), 8.99 (s, 1H), 8.52 (s, 1H), 8.39-8.51 (m, 1H), 7.68 (dd, J=9.7, 1.9 Hz, 1H), 7.47 (d, J=8.5 Hz, 2H), 7.27 (d, J=8.5 Hz, 2H), 7.14 (td, J=9.0, 2.0 Hz, 1H), 4.14 (s, 3H), 1.44 (s, 4H).

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