反应详情

温度

85 °C

To a stirred solution of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-(2-trimethylsilanylethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [1-(4-bromo-phenyl)-cyclopropyl]-amide (50 mg, 0.078 mmol) in THF (3 mL) was added a solution of TBAF (1.0 M in THF, 1.18 mL, 1.18 mmol) at room temperature. The resulting light brown solution was heated to 85° C. and stirred for 3 h at which time TLC analysis (EA, Rf=0.3) indicated the absence of starting material. Then, the reaction mixture was cooled to room temperature and 3 mL of acetone and 9 mL of saturated sodium bicarbonate solution were added and the resulting suspension was stirred for 1 h. The reaction suspension was diluted with water and the organic compound was extracted into EtOAc (3×25 mL) and the combined extracts were washed with brine solution and dried over MgSO4. Filtration of the drying agent and removal of the solvent under vacuum gave the crude solid which was dissolved in acetonitrile with heating. The resulting greenish solution was stored in the refrigerator for 15 h and the solids were collected by filtration and washed with acetonitrile. After drying in air, 25 mg (63%) of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [1-(4-bromo-phenyl)-cyclopropyl]-amide was isolated as a off-white solid. LC-MS (M+H)+=507; 1H NMR (300 MHz, DMSO-d6) δ: 12.91 (s, 1H), 9.11 (s, 1H), 8.99 (s, 1H), 8.52 (s, 1H), 8.39-8.51 (m, 1H), 7.68 (dd, J=9.7, 1.9 Hz, 1H), 7.47 (d, J=8.5 Hz, 2H), 7.27 (d, J=8.5 Hz, 2H), 7.14 (td, J=9.0, 2.0 Hz, 1H), 4.14 (s, 3H), 1.44 (s, 4H).

1. temperatureThen, the reaction mixture was cooled to room temperature
2. extractionthe organic compound was extracted into EtOAc (3×25 mL)
3. washthe combined extracts were washed with brine solution
4. dry with materialdried over MgSO4
5. filtrationFiltration of the drying agent and removal of the solvent under vacuum
6. customgave the crude solid which
7. temperaturewith heating
8. waitThe resulting greenish solution was stored in the refrigerator for 15 h
9. filtrationthe solids were collected by filtration
10. washwashed with acetonitrile
11. customAfter drying in air