Trifluoroacetic acid → N-tert-butyl-2-(5-(difluoromethoxy)-1-(2-oxo-2-(4-(2,2,2-trifluoroethyl)piperazin-1-yl)ethyl)-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide

HRID 246535Notes

Reaction equation

HRID 246535 reaction equation
[C:1]([NH:5][C:6]([C:8]1[C:16]2[C:11](=[N:12][CH:13]=[C:14]([C:17]3[C:25]4[C:20](=[CH:21][CH:22]=[C:23]([O:26][CH:27]([F:29])[F:28])[CH:24]=4)[N:19]([CH2:30][C:31](=[O:43])[N:32]4[CH2:37][CH2:36][N:35]([CH2:38][C:39]([F:42])([F:41])[F:40])[CH2:34][CH2:33]4)[N:18]=3)[N:15]=2)[N:10](COCC[Si](C)(C)C)[CH:9]=1)=[O:7])([CH3:4])([CH3:3])[CH3:2].FC(F)(F)C(O)=O>ClCCl.C(O)C>[C:1]([NH:5][C:6]([C:8]1[C:16]2[C:11](=[N:12][CH:13]=[C:14]([C:17]3[C:25]4[C:20](=[CH:21][CH:22]=[C:23]([O:26][CH:27]([F:28])[F:29])[CH:24]=4)[N:19]([CH2:30][C:31](=[O:43])[N:32]4[CH2:37][CH2:36][N:35]([CH2:38][C:39]([F:40])([F:41])[F:42])[CH2:34][CH2:33]4)[N:18]=3)[N:15]=2)[NH:10][CH:9]=1)=[O:7])([CH3:4])([CH3:2])[CH3:3]

Participants

5

Reactant 2

HCID 6422Trifluoroacetic acidHCID 71280545N-tert-butyl-2-(5-(difluoromethoxy)-1-(2-oxo-2-(4-(2,2,2-trifluoroethyl)piperazin-1-yl)ethyl)-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide

Product 1

HCID 71279881N-tert-butyl-2-(5-(difluoromethoxy)-1-(2-oxo-2-(4-(2,2,2-trifluoroethyl)piperazin-1-yl)ethyl)-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamideYield 53%

Reagents, catalysts & solvents 2

HCID 702EthanolSolventHCID 6344DichloromethaneSolvent

Conditions

Temperature
25 °C

Procedure

To a stirred solution of N-tert-butyl-2-(5-(difluoromethoxy)-1-(2-oxo-2-(4-(2,2,2-trifluoroethyl)piperazin-1-yl)ethyl)-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (75.9 mg, 103 μmol) in dichloromethane (5 mL) under Ar, trifluoroacetic acid (744 mg, 0.50 mL, 6.52 mmol) was added at 25° C. After 96 h, the reaction was concentrated in vacuo and stirred in a mixture of dichloromethane/methanol/NH3OH (3:2:0.25, 5.25 mL) at 25° C. After 2.5 h, the material was concentrated and dried in vacuo (200 mTorr, 25° C.) to give a yellow solid. The solid was purified by chromatography (silica, 12 g Analogix column, 10-100% ethyl acetate in hexanes, gradient over 1 h) to give a yellow oil. The oil was dissolved in ethanol (4.5 mL, 200 proof) and cooled to 4° C. The precipitated solid was isolated by filtration and dried in vacuo (100 mTorr, 40° C.) to give N-tert-butyl-2-(5-(difluoromethoxy)-1-(2-oxo-2-(4-(2,2,2-trifluoroethyl)piperazin-1-yl)ethyl)-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (36.2 mg, 54.1 μmol, 53%) as a yellow solid. MS (M)'=609; 1H NMR (DMSO-d6) δ: 12.84 (d, J=3.0 Hz, 1H), 9.03 (s, 1H), 8.40 (d, J=3.0 Hz, 1H), 8.21 (d, J=2.3 Hz, 1H), 7.89 (s, 1H), 7.77 (d, J=9.0 Hz, 1H), 7.39 (dd, J=2.3, 9.0 Hz, 1H), 7.20 (t, J=74.5 Hz, 1H), 5.66 (s, 2H), 3.64 (br. s, 2H), 3.47 (br. s, 2H), 3.29 (q, J=10.3 Hz, 2H), 2.77 (br. s, 2H), 2.64 (br. s, 2H), 1.51 (s, 9H).

Workup

  1. concentrationthe reaction was concentrated in vacuo
  2. waitAfter 2.5 h
  3. concentrationthe material was concentrated
  4. customdried in vacuo (200 mTorr, 25° C.)
  5. customto give a yellow solid
  6. customThe solid was purified by chromatography (silica, 12 g Analogix column, 10-100% ethyl acetate in hexanes, gradient over 1 h)
  7. customto give a yellow oil
  8. temperaturecooled to 4° C
  9. customThe precipitated solid was isolated by filtration
  10. customdried in vacuo (100 mTorr, 40° C.)

Sources & evidence

ORD record
ord-ada4df0e208d4908a1754117bcedea1b
Source dataset
uspto-grants-2014_02
Patent number
US08658646B2

Reaction notes

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