反应详情

To a solution of N-(1-(tert-butyldimethylsilyloxy)-2-methylpropan-2-yl)-2-(6-fluoro-1-(3-oxobutyl)-1H-indazol-3-yl)-5-trityl-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide (40 mg, 0.05 mmol) in MeOH (5 mL) was added NaBH4 (10 mg, 0.25 mmol) at 0° C. The mixture was stirred at room temperature for 1 hour. NH4Cl (aqueous) was added to the mixture to quench the reaction. The mixture was extracted with EtOAc (3×5 mL). The combined organic layers were washed with brine and dried over Na2SO4. After filtration and concentration, the crude was used into the next step without purification. LCMS: (M+H)+=797.

1. customto quench
2. customthe reaction
3. extractionThe mixture was extracted with EtOAc (3×5 mL)
4. washThe combined organic layers were washed with brine
5. dry with materialdried over Na2SO4
6. filtrationAfter filtration and concentration
7. customthe crude was used into the next step without purification